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pgem-t easy a1360  (Promega)

 
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    Structured Review

    Promega pgem-t easy a1360
    Pgem T Easy A1360, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pgem-t easy a1360/product/Promega
    Average 90 stars, based on 1 article reviews
    pgem-t easy a1360 - by Bioz Stars, 2026-04
    90/100 stars

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    pTYAa01, designed to generate a cdtABC knockout with a kanamycin-resistance cassette via homologous recombination. (B) pTYAa02, designed to generate a cdtABC knockout with a spectinomycin-resistance cassette. (C-E) pTYAa03 ( cdtA -His 6 ), pTYAa04 ( cdtB -His 6 ), and pTYAa05 ( cdtC -His 6 ), cloned with 5’UTRs including the native promoter region of the cdtABC operon. (F) pTYAa06, designed to generate a cdtABC complement with a spectinomycin-resistance cassette via homologous recombination.

    Journal: bioRxiv

    Article Title: Periodontal pathogen-derived extracellular vesicles promote EGFR-dependent malignant traits in human pancreatic cancer cells

    doi: 10.64898/2026.01.29.700732

    Figure Lengend Snippet: pTYAa01, designed to generate a cdtABC knockout with a kanamycin-resistance cassette via homologous recombination. (B) pTYAa02, designed to generate a cdtABC knockout with a spectinomycin-resistance cassette. (C-E) pTYAa03 ( cdtA -His 6 ), pTYAa04 ( cdtB -His 6 ), and pTYAa05 ( cdtC -His 6 ), cloned with 5’UTRs including the native promoter region of the cdtABC operon. (F) pTYAa06, designed to generate a cdtABC complement with a spectinomycin-resistance cassette via homologous recombination.

    Article Snippet: Approximately 1-kb fragments flanking the km cassette and the spectinomycin-resistance cassette (amplified from pUTmini-Tn5 Sm) were assembled into NdeI-digested pGEM-T Easy (pTYAa02; ) using NEBuilder.

    Techniques: Knock-Out, Homologous Recombination, Clone Assay